Preparation of Eicosapentaenoic and Docosahexaenoic Acids by Reversed Phase High Performance Liquid Chromatography

Reversed-phase high-performance liquid chromatography.

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Determination of ginkgolic acids from Ginkgo biloba leaves by reversed-phase argentation high performance liquid chromatography.

An analytical method has been firstly achieved for the quantification of ginkgolic acids from Ginkgo biloba leaves by reversed-phase argentation high performance liquid chromatography. Analytical sample was cleaned-up after addition of acidic salt solution and adsorbent to the matrix solution by counter-extraction of analytes with hexane. Ginkgolic acids were determined by HPLC with methanol an...

Reversed-phase High-performance Liquid Chromatography and Scanning Electron Microscopy

The zein proteins of eight opaque maize mutants were examined by reversed-phase high-performance liquid chromatography (RP-HPLC) and the microstructure of their endosperms by scanning electron microscopy (SEM). The opaque (0) mutants included 01, 05, 09) 010) 011) hI, Oh43JlI and V\ 64AJll. In these opaque mutant maize kernels, the proportion (%) of ex-zein, the major component of the alcohol-e...